Construct: shRNA TRCN0000127441

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172706.1-990s1c1
DNA Barcode:
CCAGGCACTTTCAGCAACAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
9330182L06Rik (231014)
Gene Description:
RIKEN cDNA 9330182L06 gene
Transcript:
RefSeq NM_172706.1 (NON-CURRENT)
Match location:
Position 990 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127441)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGCACTTTCAGCAACAAA
Hairpin Sequence:
5'-CCGG-CCAGGCACTTTCAGCAACAAA-CTCGAG-TTTGTTGCTGAAAGTGCCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGCACTTTCAGCAACAAACTCGAGTTTGTTGCTGAAAGTGCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGCACTTTCAGCAACAAACTCGAGTTTGTTGCTGAAAGTGCCTGG-3'

Other clones with same target sequence:

(none)