Construct: shRNA TRCN0000127539

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023072.1-2817s1c1
DNA Barcode:
CCCAATGCTGTCCGATATTCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZSWIM4 (65249)
Gene Description:
zinc finger SWIM-type containing 4
Transcript:
RefSeq NM_023072.1 (NON-CURRENT)
Match location:
Position 2817 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127539)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAATGCTGTCCGATATTCT
Hairpin Sequence:
5'-CCGG-CCCAATGCTGTCCGATATTCT-CTCGAG-AGAATATCGGACAGCATTGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAATGCTGTCCGATATTCTCTCGAGAGAATATCGGACAGCATTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAATGCTGTCCGATATTCTCTCGAGAGAATATCGGACAGCATTGGG-3'

Other clones with same target sequence:

(none)