Construct: shRNA TRCN0000127560

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144977.1-1718s1c1
DNA Barcode:
CTTCCTGTCTTTCCTTCTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DENND1B (163486)
Gene Description:
DENN domain containing 1B
Transcript:
RefSeq NM_144977.1 (NON-CURRENT)
Match location:
Position 1718 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127560)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCCTGTCTTTCCTTCTGAA
Hairpin Sequence:
5'-CCGG-CTTCCTGTCTTTCCTTCTGAA-CTCGAG-TTCAGAAGGAAAGACAGGAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCCTGTCTTTCCTTCTGAACTCGAGTTCAGAAGGAAAGACAGGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCCTGTCTTTCCTTCTGAACTCGAGTTCAGAAGGAAAGACAGGAAG-3'

Other clones with same target sequence:

(none)