Construct: shRNA TRCN0000127567

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152410.1-1192s1c1
DNA Barcode:
CCTTGTGACTTCCACAGCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PACRG (135138)
Gene Description:
parkin coregulated
Transcript:
RefSeq NM_152410.1 (NON-CURRENT)
Match location:
Position 1192 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127567)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGTGACTTCCACAGCTTT
Hairpin Sequence:
5'-CCGG-CCTTGTGACTTCCACAGCTTT-CTCGAG-AAAGCTGTGGAAGTCACAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGTGACTTCCACAGCTTTCTCGAGAAAGCTGTGGAAGTCACAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGTGACTTCCACAGCTTTCTCGAGAAAGCTGTGGAAGTCACAAGG-3'

Other clones with same target sequence:

(none)