Construct: shRNA TRCN0000127622

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145047.3-1326s1c1
DNA Barcode:
CCAGAATGATGCTGCTAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OSCP1 (127700)
Gene Description:
organic solute carrier partner 1
Transcript:
RefSeq NM_145047.3 (NON-CURRENT)
Match location:
Position 1326 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127622)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAATGATGCTGCTAGTTT
Hairpin Sequence:
5'-CCGG-CCAGAATGATGCTGCTAGTTT-CTCGAG-AAACTAGCAGCATCATTCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAATGATGCTGCTAGTTTCTCGAGAAACTAGCAGCATCATTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAATGATGCTGCTAGTTTCTCGAGAAACTAGCAGCATCATTCTGG-3'

Other clones with same target sequence:

(none)