Construct: shRNA TRCN0000127702

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_052958.1-3047s1c1
DNA Barcode:
GAGAACACTAATCCTGGCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C8orf34 (116328)
Gene Description:
chromosome 8 open reading frame 34
Transcript:
RefSeq NM_052958.1 (NON-CURRENT)
Match location:
Position 3047 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127702)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAACACTAATCCTGGCAAA
Hairpin Sequence:
5'-CCGG-GAGAACACTAATCCTGGCAAA-CTCGAG-TTTGCCAGGATTAGTGTTCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAACACTAATCCTGGCAAACTCGAGTTTGCCAGGATTAGTGTTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAACACTAATCCTGGCAAACTCGAGTTTGCCAGGATTAGTGTTCTC-3'

Other clones with same target sequence:

(none)