Construct: shRNA TRCN0000127711
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_014615.1-5534s1c1
- DNA Barcode:
- GCCAGTATTATCAGCAGTCAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GSE1 (23199)
- Gene Description:
- Gse1 coiled-coil protein
- Transcript:
- RefSeq NM_014615.1 (NON-CURRENT)
- Match location:
- Position 5534 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000127711)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCAGTATTATCAGCAGTCAA
- Hairpin Sequence:
-
5'-CCGG-GCCAGTATTATCAGCAGTCAA-CTCGAG-TTGACTGCTGATAATACTGGC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCAGTATTATCAGCAGTCAACTCGAGTTGACTGCTGATAATACTGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCAGTATTATCAGCAGTCAACTCGAGTTGACTGCTGATAATACTGGC-3'
Other clones with same target sequence:
(none)
