Construct: shRNA TRCN0000127760

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014706.2-316s1c1
DNA Barcode:
GCAGTTGTCTATCAACGTCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SART3 (9733)
Gene Description:
spliceosome associated factor 3, U4/U6 recycling protein
Transcript:
RefSeq NM_014706.2 (NON-CURRENT)
Match location:
Position 316 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127760)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTTGTCTATCAACGTCTA
Hairpin Sequence:
5'-CCGG-GCAGTTGTCTATCAACGTCTA-CTCGAG-TAGACGTTGATAGACAACTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTTGTCTATCAACGTCTACTCGAGTAGACGTTGATAGACAACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTTGTCTATCAACGTCTACTCGAGTAGACGTTGATAGACAACTGC-3'

Other clones with same target sequence:

(none)