Construct: shRNA TRCN0000127800

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173609.1-765s1c1
DNA Barcode:
GTGTGAAGAAGAAGAGGCGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HMGN2P46 (283651)
Gene Description:
high mobility group nucleosomal binding domain 2 pseudogene 46
Transcript:
RefSeq NM_173609.1 (NON-CURRENT)
Match location:
Position 765 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127800)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTGAAGAAGAAGAGGCGAT
Hairpin Sequence:
5'-CCGG-GTGTGAAGAAGAAGAGGCGAT-CTCGAG-ATCGCCTCTTCTTCTTCACAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTGAAGAAGAAGAGGCGATCTCGAGATCGCCTCTTCTTCTTCACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTGAAGAAGAAGAGGCGATCTCGAGATCGCCTCTTCTTCTTCACAC-3'

Other clones with same target sequence:

(none)