Construct: shRNA TRCN0000127815

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017447.2-969s1c1
DNA Barcode:
GAAGAAAGTTGTGGTCCGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C21orf91 (54149)
Gene Description:
chromosome 21 open reading frame 91
Transcript:
RefSeq NM_017447.2 (NON-CURRENT)
Match location:
Position 969 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127815)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGAAAGTTGTGGTCCGTTT
Hairpin Sequence:
5'-CCGG-GAAGAAAGTTGTGGTCCGTTT-CTCGAG-AAACGGACCACAACTTTCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGAAAGTTGTGGTCCGTTTCTCGAGAAACGGACCACAACTTTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGAAAGTTGTGGTCCGTTTCTCGAGAAACGGACCACAACTTTCTTC-3'

Other clones with same target sequence:

  1. TRCN0000312503