Construct: shRNA TRCN0000127818

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024993.3-1149s1c1
DNA Barcode:
GCCAAGGTTTGACATGGACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRRTM4 (80059)
Gene Description:
leucine rich repeat transmembrane neuronal 4
Transcript:
RefSeq NM_024993.3 (NON-CURRENT)
Match location:
Position 1149 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127818)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGGTTTGACATGGACTT
Hairpin Sequence:
5'-CCGG-GCCAAGGTTTGACATGGACTT-CTCGAG-AAGTCCATGTCAAACCTTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGGTTTGACATGGACTTCTCGAGAAGTCCATGTCAAACCTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGGTTTGACATGGACTTCTCGAGAAGTCCATGTCAAACCTTGGC-3'

Other clones with same target sequence:

(none)