Construct: shRNA TRCN0000127824

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181719.2-769s1c1
DNA Barcode:
GCATCCCGAAAGAAGAAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMCO4 (255104)
Gene Description:
transmembrane and coiled-coil domains 4
Transcript:
RefSeq NM_181719.2 (NON-CURRENT)
Match location:
Position 769 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127824)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCCCGAAAGAAGAAAGAA
Hairpin Sequence:
5'-CCGG-GCATCCCGAAAGAAGAAAGAA-CTCGAG-TTCTTTCTTCTTTCGGGATGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCCCGAAAGAAGAAAGAACTCGAGTTCTTTCTTCTTTCGGGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCCCGAAAGAAGAAAGAACTCGAGTTCTTTCTTCTTTCGGGATGC-3'

Other clones with same target sequence:

(none)