Construct: shRNA TRCN0000127904

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004816.2-2192s1c1
DNA Barcode:
CTCTGCGATTTGGCAGGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM189A2 (9413)
Gene Description:
family with sequence similarity 189 member A2
Transcript:
RefSeq NM_004816.2 (NON-CURRENT)
Match location:
Position 2192 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127904)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTGCGATTTGGCAGGAAAT
Hairpin Sequence:
5'-CCGG-CTCTGCGATTTGGCAGGAAAT-CTCGAG-ATTTCCTGCCAAATCGCAGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTGCGATTTGGCAGGAAATCTCGAGATTTCCTGCCAAATCGCAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTGCGATTTGGCAGGAAATCTCGAGATTTCCTGCCAAATCGCAGAG-3'

Other clones with same target sequence:

  1. TRCN0000275874