Construct: shRNA TRCN0000127971

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004816.2-619s1c1
DNA Barcode:
GCCTTGAAACTCTTTCCGGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM189A2 (9413)
Gene Description:
family with sequence similarity 189 member A2
Transcript:
RefSeq NM_004816.2 (NON-CURRENT)
Match location:
Position 619 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127971)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTGAAACTCTTTCCGGTT
Hairpin Sequence:
5'-CCGG-GCCTTGAAACTCTTTCCGGTT-CTCGAG-AACCGGAAAGAGTTTCAAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTGAAACTCTTTCCGGTTCTCGAGAACCGGAAAGAGTTTCAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTGAAACTCTTTCCGGTTCTCGAGAACCGGAAAGAGTTTCAAGGC-3'

Other clones with same target sequence:

  1. TRCN0000275871