Construct: shRNA TRCN0000127973

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014846.2-851s1c1
DNA Barcode:
GCTGTACGTGATTCTCTACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WASHC5 (9897)
Gene Description:
WASH complex subunit 5
Transcript:
RefSeq NM_014846.2 (NON-CURRENT)
Match location:
Position 851 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127973)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTACGTGATTCTCTACTT
Hairpin Sequence:
5'-CCGG-GCTGTACGTGATTCTCTACTT-CTCGAG-AAGTAGAGAATCACGTACAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTACGTGATTCTCTACTTCTCGAGAAGTAGAGAATCACGTACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTACGTGATTCTCTACTTCTCGAGAAGTAGAGAATCACGTACAGC-3'

Other clones with same target sequence:

(none)