Construct: shRNA TRCN0000127986

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020187.1-472s1c1
DNA Barcode:
GATTCTATGAGTGGCAGCGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HMCES (56941)
Gene Description:
5-hydroxymethylcytosine binding, ES cell specific
Transcript:
RefSeq NM_020187.1 (NON-CURRENT)
Match location:
Position 472 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127986)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTCTATGAGTGGCAGCGAT
Hairpin Sequence:
5'-CCGG-GATTCTATGAGTGGCAGCGAT-CTCGAG-ATCGCTGCCACTCATAGAATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTCTATGAGTGGCAGCGATCTCGAGATCGCTGCCACTCATAGAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTCTATGAGTGGCAGCGATCTCGAGATCGCTGCCACTCATAGAATC-3'

Other clones with same target sequence:

(none)