Construct: shRNA TRCN0000127987

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022978.1-254s1c1
DNA Barcode:
GAAAGAGGAAAGAGGATAGCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SERF1B (728492)
Gene Description:
small EDRK-rich factor 1B
Transcript:
RefSeq NM_022978.1 (NON-CURRENT)
Match location:
Position 254 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127987)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAAGAGGAAAGAGGATAGCT
Hairpin Sequence:
5'-CCGG-GAAAGAGGAAAGAGGATAGCT-CTCGAG-AGCTATCCTCTTTCCTCTTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAAGAGGAAAGAGGATAGCTCTCGAGAGCTATCCTCTTTCCTCTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAAGAGGAAAGAGGATAGCTCTCGAGAGCTATCCTCTTTCCTCTTTC-3'

Other clones with same target sequence:

  1. TRCN0000137176