Construct: shRNA TRCN0000128004

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022753.1-553s1c1
DNA Barcode:
GAACAGCAGAAGCAGCTTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
S100PBP (64766)
Gene Description:
S100P binding protein
Transcript:
RefSeq NM_022753.1 (NON-CURRENT)
Match location:
Position 553 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000128004)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACAGCAGAAGCAGCTTTAT
Hairpin Sequence:
5'-CCGG-GAACAGCAGAAGCAGCTTTAT-CTCGAG-ATAAAGCTGCTTCTGCTGTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACAGCAGAAGCAGCTTTATCTCGAGATAAAGCTGCTTCTGCTGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACAGCAGAAGCAGCTTTATCTCGAGATAAAGCTGCTTCTGCTGTTC-3'

Other clones with same target sequence:

  1. TRCN0000276102