Construct: shRNA TRCN0000128008

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145055.1-371s1c1
DNA Barcode:
GATACGTTGTCTTCCGCAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C18orf25 (147339)
Gene Description:
chromosome 18 open reading frame 25
Transcript:
RefSeq NM_145055.1 (NON-CURRENT)
Match location:
Position 371 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000128008)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATACGTTGTCTTCCGCAGAA
Hairpin Sequence:
5'-CCGG-GATACGTTGTCTTCCGCAGAA-CTCGAG-TTCTGCGGAAGACAACGTATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATACGTTGTCTTCCGCAGAACTCGAGTTCTGCGGAAGACAACGTATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATACGTTGTCTTCCGCAGAACTCGAGTTCTGCGGAAGACAACGTATC-3'

Other clones with same target sequence:

(none)