Construct: shRNA TRCN0000128015

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001012421.1-1859s1c1
DNA Barcode:
GCTGAGAATACAAGGCTCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANKRD20A2 (441430)
Gene Description:
ankyrin repeat domain 20 family member A2
Transcript:
RefSeq NM_001012421.1 (CURRENT)
Match location:
Position 1859 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000128015)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAGAATACAAGGCTCAAT
Hairpin Sequence:
5'-CCGG-GCTGAGAATACAAGGCTCAAT-CTCGAG-ATTGAGCCTTGTATTCTCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAGAATACAAGGCTCAATCTCGAGATTGAGCCTTGTATTCTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAGAATACAAGGCTCAATCTCGAGATTGAGCCTTGTATTCTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000136726