Construct: shRNA TRCN0000128033

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145032.2-2479s1c2
DNA Barcode:
GCAGCAGGAATACAACACTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FBXL13 (222235)
Gene Description:
F-box and leucine rich repeat protein 13
Transcript:
RefSeq NM_145032.2 (NON-CURRENT)
Match location:
Position 2479 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000128033)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCAGGAATACAACACTAA
Hairpin Sequence:
5'-CCGG-GCAGCAGGAATACAACACTAA-CTCGAG-TTAGTGTTGTATTCCTGCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCAGGAATACAACACTAACTCGAGTTAGTGTTGTATTCCTGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCAGGAATACAACACTAACTCGAGTTAGTGTTGTATTCCTGCTGC-3'

Other clones with same target sequence:

  1. TRCN0000118583