Construct: shRNA TRCN0000128047

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145032.2-1191s1c1
DNA Barcode:
GCCCAACATTCACAGATGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FBXL13 (222235)
Gene Description:
F-box and leucine rich repeat protein 13
Transcript:
RefSeq NM_145032.2 (NON-CURRENT)
Match location:
Position 1191 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000128047)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAACATTCACAGATGAAT
Hairpin Sequence:
5'-CCGG-GCCCAACATTCACAGATGAAT-CTCGAG-ATTCATCTGTGAATGTTGGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAACATTCACAGATGAATCTCGAGATTCATCTGTGAATGTTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAACATTCACAGATGAATCTCGAGATTCATCTGTGAATGTTGGGC-3'

Other clones with same target sequence:

(none)