Construct: shRNA TRCN0000128070

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016333.2-4060s1c1
DNA Barcode:
GCTGTGTCTTTGACTCTTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SRRM2 (23524)
Gene Description:
serine/arginine repetitive matrix 2
Transcript:
RefSeq NM_016333.2 (NON-CURRENT)
Match location:
Position 4060 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000128070)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTGTCTTTGACTCTTGAT
Hairpin Sequence:
5'-CCGG-GCTGTGTCTTTGACTCTTGAT-CTCGAG-ATCAAGAGTCAAAGACACAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTGTCTTTGACTCTTGATCTCGAGATCAAGAGTCAAAGACACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTGTCTTTGACTCTTGATCTCGAGATCAAGAGTCAAAGACACAGC-3'

Other clones with same target sequence:

(none)