Construct: shRNA TRCN0000128148

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183387.1-625s1c1
DNA Barcode:
CCTGGTCATACAGATAGAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EML5 (161436)
Gene Description:
EMAP like 5
Transcript:
RefSeq NM_183387.1 (NON-CURRENT)
Match location:
Position 625 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000128148)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGTCATACAGATAGAATA
Hairpin Sequence:
5'-CCGG-CCTGGTCATACAGATAGAATA-CTCGAG-TATTCTATCTGTATGACCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGTCATACAGATAGAATACTCGAGTATTCTATCTGTATGACCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGTCATACAGATAGAATACTCGAGTATTCTATCTGTATGACCAGG-3'

Other clones with same target sequence:

(none)