Construct: shRNA TRCN0000128158

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014810.2-4017s1c1
DNA Barcode:
CCTCCAACTATAACAGGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CEP350 (9857)
Gene Description:
centrosomal protein 350
Transcript:
RefSeq NM_014810.2 (NON-CURRENT)
Match location:
Position 4017 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000128158)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCAACTATAACAGGATTT
Hairpin Sequence:
5'-CCGG-CCTCCAACTATAACAGGATTT-CTCGAG-AAATCCTGTTATAGTTGGAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCAACTATAACAGGATTTCTCGAGAAATCCTGTTATAGTTGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCAACTATAACAGGATTTCTCGAGAAATCCTGTTATAGTTGGAGG-3'

Other clones with same target sequence:

  1. TRCN0000330114