Construct: shRNA TRCN0000128253

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198520.1-735s1c1
DNA Barcode:
GCTGAGAATACAGAAGTTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C12orf63 (374467)
Gene Description:
chromosome 12 open reading frame 63
Transcript:
RefSeq NM_198520.1 (NON-CURRENT)
Match location:
Position 735 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000128253)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAGAATACAGAAGTTCAA
Hairpin Sequence:
5'-CCGG-GCTGAGAATACAGAAGTTCAA-CTCGAG-TTGAACTTCTGTATTCTCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAGAATACAGAAGTTCAACTCGAGTTGAACTTCTGTATTCTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAGAATACAGAAGTTCAACTCGAGTTGAACTTCTGTATTCTCAGC-3'

Other clones with same target sequence:

(none)