Construct: shRNA TRCN0000128254

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_203308.1-494s1c1
DNA Barcode:
GAAGATTCATCACTAAGCCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPL13AP17 (399670)
Gene Description:
ribosomal protein L13a pseudogene 17
Transcript:
RefSeq NM_203308.1 (NON-CURRENT)
Match location:
Position 494 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000128254)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGATTCATCACTAAGCCAT
Hairpin Sequence:
5'-CCGG-GAAGATTCATCACTAAGCCAT-CTCGAG-ATGGCTTAGTGATGAATCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGATTCATCACTAAGCCATCTCGAGATGGCTTAGTGATGAATCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGATTCATCACTAAGCCATCTCGAGATGGCTTAGTGATGAATCTTC-3'

Other clones with same target sequence:

(none)