Construct: shRNA TRCN0000128328

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018249.3-2348s1c1
DNA Barcode:
GCAGCAGAGTAATGAGATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDK5RAP2 (55755)
Gene Description:
CDK5 regulatory subunit associated protein 2
Transcript:
RefSeq NM_018249.3 (NON-CURRENT)
Match location:
Position 2348 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000128328)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCAGAGTAATGAGATTAT
Hairpin Sequence:
5'-CCGG-GCAGCAGAGTAATGAGATTAT-CTCGAG-ATAATCTCATTACTCTGCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCAGAGTAATGAGATTATCTCGAGATAATCTCATTACTCTGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCAGAGTAATGAGATTATCTCGAGATAATCTCATTACTCTGCTGC-3'

Other clones with same target sequence:

(none)