Construct: shRNA TRCN0000128399

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153610.2-914s1c1
DNA Barcode:
GAAGAGAAACTCTCAAAGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CMYA5 (202333)
Gene Description:
cardiomyopathy associated 5
Transcript:
RefSeq NM_153610.2 (NON-CURRENT)
Match location:
Position 914 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000128399)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGAGAAACTCTCAAAGGAA
Hairpin Sequence:
5'-CCGG-GAAGAGAAACTCTCAAAGGAA-CTCGAG-TTCCTTTGAGAGTTTCTCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGAGAAACTCTCAAAGGAACTCGAGTTCCTTTGAGAGTTTCTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGAGAAACTCTCAAAGGAACTCGAGTTCCTTTGAGAGTTTCTCTTC-3'

Other clones with same target sequence:

(none)