Construct: shRNA TRCN0000128516

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018442.1-1536s1c1
DNA Barcode:
CAGAAACTTCAGATCAGACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DCAF6 (55827)
Gene Description:
DDB1 and CUL4 associated factor 6
Transcript:
RefSeq NM_018442.1 (NON-CURRENT)
Match location:
Position 1536 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000128516)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAAACTTCAGATCAGACTA
Hairpin Sequence:
5'-CCGG-CAGAAACTTCAGATCAGACTA-CTCGAG-TAGTCTGATCTGAAGTTTCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAAACTTCAGATCAGACTACTCGAGTAGTCTGATCTGAAGTTTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAAACTTCAGATCAGACTACTCGAGTAGTCTGATCTGAAGTTTCTG-3'

Other clones with same target sequence:

  1. TRCN0000352974