Construct: shRNA TRCN0000128543

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020458.1-1597s1c1
DNA Barcode:
CGTTTGGAGAATTTCACCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TTC7A (57217)
Gene Description:
tetratricopeptide repeat domain 7A
Transcript:
RefSeq NM_020458.1 (NON-CURRENT)
Match location:
Position 1597 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000128543)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTTGGAGAATTTCACCTTT
Hairpin Sequence:
5'-CCGG-CGTTTGGAGAATTTCACCTTT-CTCGAG-AAAGGTGAAATTCTCCAAACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTTGGAGAATTTCACCTTTCTCGAGAAAGGTGAAATTCTCCAAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTTGGAGAATTTCACCTTTCTCGAGAAAGGTGAAATTCTCCAAACG-3'

Other clones with same target sequence:

(none)