Construct: shRNA TRCN0000128545

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014723.2-2740s1c1
DNA Barcode:
GCTTTGCACTATGTTCACTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SNPH (9751)
Gene Description:
syntaphilin
Transcript:
RefSeq NM_014723.2 (NON-CURRENT)
Match location:
Position 2740 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000128545)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTGCACTATGTTCACTTT
Hairpin Sequence:
5'-CCGG-GCTTTGCACTATGTTCACTTT-CTCGAG-AAAGTGAACATAGTGCAAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTGCACTATGTTCACTTTCTCGAGAAAGTGAACATAGTGCAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTGCACTATGTTCACTTTCTCGAGAAAGTGAACATAGTGCAAAGC-3'

Other clones with same target sequence:

(none)