Construct: shRNA TRCN0000128602

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014753.2-4003s1c1
DNA Barcode:
CAAGAGATGTCTCTACTCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BMS1 (9790)
Gene Description:
BMS1 ribosome biogenesis factor
Transcript:
RefSeq NM_014753.2 (NON-CURRENT)
Match location:
Position 4003 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000128602)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGAGATGTCTCTACTCAAA
Hairpin Sequence:
5'-CCGG-CAAGAGATGTCTCTACTCAAA-CTCGAG-TTTGAGTAGAGACATCTCTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGAGATGTCTCTACTCAAACTCGAGTTTGAGTAGAGACATCTCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGAGATGTCTCTACTCAAACTCGAGTTTGAGTAGAGACATCTCTTG-3'

Other clones with same target sequence:

  1. TRCN0000297195