Construct: shRNA TRCN0000128608

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020211.1-842s1c1
DNA Barcode:
CGACAATAATTACCTGAACGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RGMA (56963)
Gene Description:
repulsive guidance molecule BMP co-receptor a
Transcript:
RefSeq NM_020211.1 (NON-CURRENT)
Match location:
Position 842 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000128608)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACAATAATTACCTGAACGT
Hairpin Sequence:
5'-CCGG-CGACAATAATTACCTGAACGT-CTCGAG-ACGTTCAGGTAATTATTGTCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACAATAATTACCTGAACGTCTCGAGACGTTCAGGTAATTATTGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACAATAATTACCTGAACGTCTCGAGACGTTCAGGTAATTATTGTCG-3'

Other clones with same target sequence:

(none)