Construct: shRNA TRCN0000128609

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020893.1-4290s1c1
DNA Barcode:
CAACAGCTTCAGAACAAGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SUGT1P4-STRA6LP-CCDC180 (57653)
Gene Description:
SUGT1P4-STRA6LP-CCDC180 readthrough
Transcript:
RefSeq NM_020893.1 (NON-CURRENT)
Match location:
Position 4290 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000128609)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACAGCTTCAGAACAAGATA
Hairpin Sequence:
5'-CCGG-CAACAGCTTCAGAACAAGATA-CTCGAG-TATCTTGTTCTGAAGCTGTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACAGCTTCAGAACAAGATACTCGAGTATCTTGTTCTGAAGCTGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACAGCTTCAGAACAAGATACTCGAGTATCTTGTTCTGAAGCTGTTG-3'

Other clones with same target sequence:

(none)