Construct: shRNA TRCN0000128629

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017760.4-3076s1c1
DNA Barcode:
CATGAGTTCATTACTGCTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NCAPG2 (54892)
Gene Description:
non-SMC condensin II complex subunit G2
Transcript:
RefSeq NM_017760.4 (NON-CURRENT)
Match location:
Position 3076 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000128629)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGAGTTCATTACTGCTGTT
Hairpin Sequence:
5'-CCGG-CATGAGTTCATTACTGCTGTT-CTCGAG-AACAGCAGTAATGAACTCATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGAGTTCATTACTGCTGTTCTCGAGAACAGCAGTAATGAACTCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGAGTTCATTACTGCTGTTCTCGAGAACAGCAGTAATGAACTCATG-3'

Other clones with same target sequence:

  1. TRCN0000322736