Construct: shRNA TRCN0000128666

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001012421.1-483s1c1
DNA Barcode:
CCAATCCAAACCTTAAGGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANKRD20A2 (441430)
Gene Description:
ankyrin repeat domain 20 family member A2
Transcript:
RefSeq NM_001012421.1 (CURRENT)
Match location:
Position 483 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000128666)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAATCCAAACCTTAAGGATA
Hairpin Sequence:
5'-CCGG-CCAATCCAAACCTTAAGGATA-CTCGAG-TATCCTTAAGGTTTGGATTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAATCCAAACCTTAAGGATACTCGAGTATCCTTAAGGTTTGGATTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAATCCAAACCTTAAGGATACTCGAGTATCCTTAAGGTTTGGATTGG-3'

Other clones with same target sequence:

  1. TRCN0000133759