Construct: shRNA TRCN0000128756

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001002255.1-246s1c1
DNA Barcode:
CAAACCTGCACAGTTGGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SUMO4 (387082)
Gene Description:
small ubiquitin like modifier 4
Transcript:
RefSeq NM_001002255.1 (NON-CURRENT)
Match location:
Position 246 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000128756)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAACCTGCACAGTTGGAAAT
Hairpin Sequence:
5'-CCGG-CAAACCTGCACAGTTGGAAAT-CTCGAG-ATTTCCAACTGTGCAGGTTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAACCTGCACAGTTGGAAATCTCGAGATTTCCAACTGTGCAGGTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAACCTGCACAGTTGGAAATCTCGAGATTTCCAACTGTGCAGGTTTG-3'

Other clones with same target sequence:

(none)