Construct: shRNA TRCN0000128818

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014931.2-3017s1c1
DNA Barcode:
GAGAGAATGGAGAGAGAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP6R1 (22870)
Gene Description:
protein phosphatase 6 regulatory subunit 1
Transcript:
RefSeq NM_014931.2 (NON-CURRENT)
Match location:
Position 3017 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000128818)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAGAATGGAGAGAGAGAAA
Hairpin Sequence:
5'-CCGG-GAGAGAATGGAGAGAGAGAAA-CTCGAG-TTTCTCTCTCTCCATTCTCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAGAATGGAGAGAGAGAAACTCGAGTTTCTCTCTCTCCATTCTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAGAATGGAGAGAGAGAAACTCGAGTTTCTCTCTCTCCATTCTCTC-3'

Other clones with same target sequence:

(none)