Construct: shRNA TRCN0000128837
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_374907.1-463s1c1
- DNA Barcode:
- GCCAAACATCAAGCAGAATGT
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- FCHSD2 (9873)
- Gene Description:
- FCH and double SH3 domains 2
- Transcript:
- RefSeq XM_374907.1 (NON-CURRENT)
- Match location:
- Position 463 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this shRNA:
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Sequence Information
- Target Sequence:
- GCCAAACATCAAGCAGAATGT
- Hairpin Sequence:
- 5'-CCGG-GCCAAACATCAAGCAGAATGT-CTCGAG-ACATTCTGCTTGATGTTTGGC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCAAACATCAAGCAGAATGTCTCGAGACATTCTGCTTGATGTTTGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCAAACATCAAGCAGAATGTCTCGAGACATTCTGCTTGATGTTTGGC-3'