Construct: shRNA TRCN0000128862

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001005527.1-658s1c1
DNA Barcode:
GATTCAGAAATGGTGGTGTCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TAFA4 (151647)
Gene Description:
TAFA chemokine like family member 4
Transcript:
RefSeq NM_001005527.1 (NON-CURRENT)
Match location:
Position 658 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000128862)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTCAGAAATGGTGGTGTCA
Hairpin Sequence:
5'-CCGG-GATTCAGAAATGGTGGTGTCA-CTCGAG-TGACACCACCATTTCTGAATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTCAGAAATGGTGGTGTCACTCGAGTGACACCACCATTTCTGAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTCAGAAATGGTGGTGTCACTCGAGTGACACCACCATTTCTGAATC-3'

Other clones with same target sequence:

(none)