Construct: shRNA TRCN0000129008

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031920.2-1415s1c1
DNA Barcode:
GCTTTGCAGATTTACCAGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMTC1 (83857)
Gene Description:
transmembrane O-mannosyltransferase targeting cadherins 1
Transcript:
RefSeq NM_031920.2 (NON-CURRENT)
Match location:
Position 1415 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000129008)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTGCAGATTTACCAGGAA
Hairpin Sequence:
5'-CCGG-GCTTTGCAGATTTACCAGGAA-CTCGAG-TTCCTGGTAAATCTGCAAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTGCAGATTTACCAGGAACTCGAGTTCCTGGTAAATCTGCAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTGCAGATTTACCAGGAACTCGAGTTCCTGGTAAATCTGCAAAGC-3'

Other clones with same target sequence:

(none)