Construct: shRNA TRCN0000129012

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145284.3-2886s1c1
DNA Barcode:
GCATCCGATGTGTCCTTTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM122B (159090)
Gene Description:
family with sequence similarity 122B
Transcript:
RefSeq NM_145284.3 (NON-CURRENT)
Match location:
Position 2886 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000129012)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCCGATGTGTCCTTTATT
Hairpin Sequence:
5'-CCGG-GCATCCGATGTGTCCTTTATT-CTCGAG-AATAAAGGACACATCGGATGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCCGATGTGTCCTTTATTCTCGAGAATAAAGGACACATCGGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCCGATGTGTCCTTTATTCTCGAGAATAAAGGACACATCGGATGC-3'

Other clones with same target sequence:

(none)