Construct: shRNA TRCN0000129022

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_371826.1-594s1c1
DNA Barcode:
GCCATACGGTTCTTCTATCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GFRAL (389400)
Gene Description:
GDNF family receptor alpha like
Transcript:
RefSeq XM_371826.1 (NON-CURRENT)
Match location:
Position 594 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000129022)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATACGGTTCTTCTATCAA
Hairpin Sequence:
5'-CCGG-GCCATACGGTTCTTCTATCAA-CTCGAG-TTGATAGAAGAACCGTATGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATACGGTTCTTCTATCAACTCGAGTTGATAGAAGAACCGTATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATACGGTTCTTCTATCAACTCGAGTTGATAGAAGAACCGTATGGC-3'

Other clones with same target sequence:

(none)