Construct: shRNA TRCN0000129045

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175056.1-286s1c1
DNA Barcode:
GCAGGCTATTACGATGAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZPLD1 (131368)
Gene Description:
zona pellucida like domain containing 1
Transcript:
RefSeq NM_175056.1 (NON-CURRENT)
Match location:
Position 286 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000129045)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGCTATTACGATGAAGAT
Hairpin Sequence:
5'-CCGG-GCAGGCTATTACGATGAAGAT-CTCGAG-ATCTTCATCGTAATAGCCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGCTATTACGATGAAGATCTCGAGATCTTCATCGTAATAGCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGCTATTACGATGAAGATCTCGAGATCTTCATCGTAATAGCCTGC-3'

Other clones with same target sequence:

(none)