Construct: shRNA TRCN0000129046

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178547.2-305s1c1
DNA Barcode:
GACTTACAGTCTCTTCTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZBTB8OS (339487)
Gene Description:
zinc finger and BTB domain containing 8 opposite strand
Transcript:
RefSeq NM_178547.2 (NON-CURRENT)
Match location:
Position 305 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000129046)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTTACAGTCTCTTCTGTTT
Hairpin Sequence:
5'-CCGG-GACTTACAGTCTCTTCTGTTT-CTCGAG-AAACAGAAGAGACTGTAAGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTTACAGTCTCTTCTGTTTCTCGAGAAACAGAAGAGACTGTAAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTTACAGTCTCTTCTGTTTCTCGAGAAACAGAAGAGACTGTAAGTC-3'

Other clones with same target sequence:

(none)