Construct: shRNA TRCN0000129075

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_034570.5-3947s1c1
DNA Barcode:
GCTCATCTGTTCTCTCCTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CRAMP1 (57585)
Gene Description:
cramped chromatin regulator homolog 1
Transcript:
RefSeq XM_034570.5 (NON-CURRENT)
Match location:
Position 3947 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000129075)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCATCTGTTCTCTCCTTAT
Hairpin Sequence:
5'-CCGG-GCTCATCTGTTCTCTCCTTAT-CTCGAG-ATAAGGAGAGAACAGATGAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCATCTGTTCTCTCCTTATCTCGAGATAAGGAGAGAACAGATGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCATCTGTTCTCTCCTTATCTCGAGATAAGGAGAGAACAGATGAGC-3'

Other clones with same target sequence:

(none)