Construct: shRNA TRCN0000129154

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017760.4-4749s1c1
DNA Barcode:
GCTTCTCAAATTGCTGGGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NCAPG2 (54892)
Gene Description:
non-SMC condensin II complex subunit G2
Transcript:
RefSeq NM_017760.4 (NON-CURRENT)
Match location:
Position 4749 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000129154)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCTCAAATTGCTGGGATT
Hairpin Sequence:
5'-CCGG-GCTTCTCAAATTGCTGGGATT-CTCGAG-AATCCCAGCAATTTGAGAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCTCAAATTGCTGGGATTCTCGAGAATCCCAGCAATTTGAGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCTCAAATTGCTGGGATTCTCGAGAATCCCAGCAATTTGAGAAGC-3'

Other clones with same target sequence:

(none)