Construct: shRNA TRCN0000129161

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032916.3-225s1c1
DNA Barcode:
GTGCTTTCTCTCAGAACTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM86B1 (85002)
Gene Description:
family with sequence similarity 86 member B1
Transcript:
RefSeq NM_032916.3 (NON-CURRENT)
Match location:
Position 225 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000129161)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGCTTTCTCTCAGAACTCAT
Hairpin Sequence:
5'-CCGG-GTGCTTTCTCTCAGAACTCAT-CTCGAG-ATGAGTTCTGAGAGAAAGCAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGCTTTCTCTCAGAACTCATCTCGAGATGAGTTCTGAGAGAAAGCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGCTTTCTCTCAGAACTCATCTCGAGATGAGTTCTGAGAGAAAGCAC-3'

Other clones with same target sequence:

(none)