Construct: shRNA TRCN0000129204

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016474.3-1092s1c1
DNA Barcode:
CCAGAGTAGCAAAGTAGAAGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC174 (51244)
Gene Description:
coiled-coil domain containing 174
Transcript:
RefSeq NM_016474.3 (NON-CURRENT)
Match location:
Position 1092 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000129204)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAGTAGCAAAGTAGAAGT
Hairpin Sequence:
5'-CCGG-CCAGAGTAGCAAAGTAGAAGT-CTCGAG-ACTTCTACTTTGCTACTCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAGTAGCAAAGTAGAAGTCTCGAGACTTCTACTTTGCTACTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAGTAGCAAAGTAGAAGTCTCGAGACTTCTACTTTGCTACTCTGG-3'

Other clones with same target sequence:

(none)